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For library enrichment, PCR amplification of 15 cycles was conducted, followed by 2% agarose gel recovery of target bands, TBS380 (Picogreen) quantification, and mixing onboard in proportion to the data, whereafter ridge PCR amplification on cBot was performed to generate clusters
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Safety notes: Potential side effects include daytime grogginess, mild headaches, nausea, and vertigo, typically associated with rapid administration
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