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Emerging therapies for cartilage regeneration in currently excluded 'red knee' populations | npj Regenerative Medicine

Finally, the Ni-NTA resin-bound proteins were eluted with BF + 200 mM imidazole, fractionated on a reducing 12% SDS-PA gel and probed with TRX f1 and NTRC antibodies following immunoblotting

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Correlation between the two markers across an ageing cohort doesn't establish that one drives the other, and untangling causality — does declining NAD+ availability impair antioxidant enzyme function, does oxidative damage consume NAD+-dependent repair capacity, or do both result from a shared upstream cause — remains a genuinely open question that a well-designed study needs to address explicitly rather than assume from population-level correlation alone