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RT-PCR reactions were performed in a total volume of 25 μl containing 25–100 ng of total RNA, 200 μm of each dNTP, 1.5 μm MgCl 2 , 25 pmol of each primer, 2.5 U of AMV RT and 2.5 U of TFL DNA polymerase (Access RT-PCR System, Promega)
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US$40
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